Lemon Balm and Dandelion Leaf Extracts Synergistically Protect against Carbon Tetrachloride-Induced Acute Liver Injury in Mice

Lemon balm and dandelion are commonly used medicinal herbs exhibiting numerous pharmacological activities that beneficial for human health. In this study, we explored the protective effects of a 2:1 (w/w) mixture lemon extracts (MLD) on carbon tetrachloride (CCl4)-induced acute liver injury in mice. CCl4 (0.5 mL/kg; i.p.) injection inhibited body weight gain increased relative weight. Pre-administration MLD (50–200 mg/kg) 7 days prevented these CCl4-mediated changes. addition, histopathological analysis revealed synergistically alleviated hepatocyte degeneration infiltration inflammatory cells. decreased serum aspartate aminotransferase alanine transferase reduced number cells stained positive cleaved caspase-3 poly(ADP-ribose) polymerase, suggesting protects against CCl4-induced hepatic damage via inhibition apoptosis. Moreover, attenuated lipid peroxidation protein nitrosylation by restoring impaired nuclear factor erythroid 2-related 2 mRNA levels its dependent antioxidant activities. Furthermore, tumor necrosis factor-α, interleukin-1β, interleukin-6 liver. Together, results suggest has potential preventing inhibiting apoptosis, oxidative stress, inflammation.